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flag ciap2 prk5  (Addgene inc)


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    Structured Review

    Addgene inc flag ciap2 prk5
    Flag Ciap2 Prk5, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flag+ciap2+prk5/Flag-cIAP2%2FpRK5+(Plasmid+%2327973)/pm40484249-259-48-50
    Average 90 stars, based on 9 article reviews
    flag ciap2 prk5 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Generated:

    Article Title: Steatotic liver disease induces YAP/TAZ-driven cell competition that can suppress tumor initiation.
    Article Snippet: Please cite this article as: Algueró-Nadal A, Mol H, Zoppolato E, Kowalczyk W, Sansores-García L, Soheily S, Hillen H, van Huffel L, Van Haele M, Corral-Ibáñez M, Hoffelner DK, van Grunsven LA, Roskams T, Govaere O, Moya IM, Halder G, Steatotic liver disease induces YAP/TAZ-driven cell competition that can suppress tumor initiation, Journal of Hepatology, https://doi.org/10.1016/ j.jhep.2025.06.002.

    Polymerase Chain Reaction:

    Article Title: Steatotic liver disease induces YAP/TAZ-driven cell competition that can suppress tumor initiation.
    Article Snippet: Please cite this article as: Algueró-Nadal A, Mol H, Zoppolato E, Kowalczyk W, Sansores-García L, Soheily S, Hillen H, van Huffel L, Van Haele M, Corral-Ibáñez M, Hoffelner DK, van Grunsven LA, Roskams T, Govaere O, Moya IM, Halder G, Steatotic liver disease induces YAP/TAZ-driven cell competition that can suppress tumor initiation, Journal of Hepatology, https://doi.org/10.1016/ j.jhep.2025.06.002.

    Plasmid Preparation:

    Article Title: Selective disruption of Traf1/cIAP2 interaction attenuates inflammatory responses and rheumatoid arthritis.
    Article Snippet: The human TRAF1 ORF was amplified from RAJI cells cDNA and cloned into pENTR-D-TOPO vector (Invitrogen) following manufacturer ‘s instructions then used as the template plasmid to generate all the different mutations. .. The TRAF1 mutated plasmids were sub-cloned into c-Flag pcDNA3. c-Flag pcDNA3 was a gift from Stephen Smale (Addgene plasmid # 20011) [37]; Flag-cIAP2/pRK5 was a gift from Xiaolu Yang (Addgene plasmid # 27973) [38]; pEBB HA cIAP1 was a gift from Colin Duckett (Addgene plasmid # 38232) [39]. ..

    Article Title: Caspase-10: a molecular switch from cell-autonomous apoptosis to communal cell death in response to chemotherapeutic drug treatment
    Article Snippet: Human cervix carcinoma HeLa cells (ATCC) and human breast carcinoma MDA-MB-231 cells (ATCC) were grown in Dulbecco’s modified eagle medium supplemented with 10% FBS, 100 U/ml penicillin and 100 μg/ml streptomycin. .. Flag-cIAP2/pRK5 was a gift from Xiaolu Yang (Addgene, Cambridge, MA, USA, plasmid # 27973), 36 pcdna3.1 hciap1 was a gift from Jon Ashwell (Addgene plasmid # 8311). .. 37 HA-Ubiquitin was a gift from Edward Yeh (Addgene plasmid # 18712).

    Article Title: cIAP2-mediated IGF2BP2 ubiquitination and degradation regulate cardiomyocyte apoptosis via stabilizing m 6 A-modified BAX mRNA in myocardial infarction
    Article Snippet: Finally, the blot was incubated with HRP-conjugated goat anti-rabbit or anti-mouse IgG antibody for 1 h at room temperature and then detected with ECL chemiluminescent reagent (cat. #34580, Thermo Fisher Scientific, USA). .. Flag-cIAP2/pRK5 (cat. #27973) and pRK5-HA-Ubiquitin-WT (cat. #17608) plasmids were obtained from Addgene (Cambridge, MA, USA), and pCMV-Myc-IGF2BP2 (cat. #43614) plasmid was constructed by YouBio (China). .. Plasmids were transfected into cells by using Lipofectamine® 2000 (cat. #11668–019, Thermo Fisher Scientific, USA) following the manufacturer’s instruction.

    Article Title: Caspase-10: a molecular switch from cell-autonomous apoptosis to communal cell death in response to chemotherapeutic drug treatment.
    Article Snippet: .. Flag-cIAP2/pRK5 was a gift from Xiaolu Yang (Addgene, Cambridge, MA, USA, plasmid # 27973),36 pcdna3.1 hciap1 was a gift from Jon Ashwell (Addgene plasmid # 8311).37 HA-Ubiquitin was a gift from Edward Cell Death and Differentiation Yeh (Addgene plasmid # 18712).38 Caspase-10 was purchased from Invivogen (San Diego, CA, USA). .. TRAF2, pCMV6.FADD.MYC and cFLIP were bought from Origene (Rockville, MD, USA).

    Construct:

    Article Title: cIAP2-mediated IGF2BP2 ubiquitination and degradation regulate cardiomyocyte apoptosis via stabilizing m 6 A-modified BAX mRNA in myocardial infarction
    Article Snippet: Finally, the blot was incubated with HRP-conjugated goat anti-rabbit or anti-mouse IgG antibody for 1 h at room temperature and then detected with ECL chemiluminescent reagent (cat. #34580, Thermo Fisher Scientific, USA). .. Flag-cIAP2/pRK5 (cat. #27973) and pRK5-HA-Ubiquitin-WT (cat. #17608) plasmids were obtained from Addgene (Cambridge, MA, USA), and pCMV-Myc-IGF2BP2 (cat. #43614) plasmid was constructed by YouBio (China). .. Plasmids were transfected into cells by using Lipofectamine® 2000 (cat. #11668–019, Thermo Fisher Scientific, USA) following the manufacturer’s instruction.



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